The Isolation and Purification of Siderophores from Marine Halomonas Strains

Abstract

The isolation of iron(III)-specific chelators also known as siderophores could have many beneficial properties including antimicrobial or antineoplastic functions. Three different strains of Halomonas bacteria were tested for siderophore production. All three strains were tested using ferric Chrome Azurol Sulfonate (Fe-CAS) in an agar plate. One strain tested positive for siderophore production and was grown in a low iron artificial seawater broth. The broth was centrifuged to collect the supernatant and a polystyrene resin was added to bind to the siderophores in the supernatant. Column chromatography was used to elute the siderophores from the polystyrene resin. The mobile phase was collected and then purified using reversed-phase high-performance liquid chromatography (RP-HPLC).